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KMID : 0545120170270040718
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 4 p.718 ~ p.724
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Fusion Peptide Improves Stability and Bioactivity of Single Chain Antibody against Rabies Virus
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Xi Hualong
Zhang Kaixin
Gu Tiejun
Sun Qing
Shi Linqing
Zhang Renxia
Jiang Chunlai
Kong Wei
Wu Yongge
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Abstract
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The combination of rabies immunoglobulin (RIG) with a vaccine is currently effective against rabies infections, but improvements are needed. Genetic engineering antibody technology is an attractive approach for developing novel antibodies to replace RIG. In our previous study, a single-chain variable fragment, scFv57R, against rabies virus glycoprotein was constructed. However, its inherent weak stability and short half-life compared with the parent RIG may limit its diagnostic and therapeutic application. Therefore, an acidic tail of synuclein (ATS) derived from the C-terminal acidic tail of human alpha-synuclein protein was fused to the Cterminus of scFv57R in order to help it resist adverse stress and improve the stability and halflife. The tail showed no apparent effect on the preparation procedure and affinity of the protein, nor did it change the neutralizing potency in vitro. In the ELISA test of molecular stability, the ATS fusion form of the protein, scFv57R-ATS, showed an increase in thermal stability and longer half-life in serum than scFv57R. The protection against fatal rabies virus challenge improved after fusing the tail to the scFv, which may be attributed to the improved stability. Thus, the ATS fusion approach presented here is easily implemented and can be used as a new strategy to improve the stability and half-life of engineered antibody proteins for practical applications.
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KEYWORD
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Rabies, scFv, rapid fluorescent focus inhibition test (RFFIT), acidic tail of synuclein (ATS), mouse challenge model
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